ISOLATION OF ANTIBIOTIC PRODUCING STREPTOMYCES FROM THE SOIL AS SOURCE ANTIBIOTICS ACTIVE AGAINST ANTIBIOTICRESISTANT STAPHYLOCOCCUS AUREUS

  • Type: Project
  • Department: Microbiology
  • Project ID: MCB0189
  • Access Fee: ₦5,000 ($14)
  • Pages: 60 Pages
  • Format: Microsoft Word
  • Views: 1K
  • Report This work

For more Info, call us on
+234 8130 686 500
or
+234 8093 423 853

ABSTRACT

The study involved the isolation of (2) isolates titled RMN1, RMN2,  belonging to the Genus Serratia  from ( 2) soil samples collected from the following two strategic locations in university of Abuja, permanent site: Termite hill besides Girls hostel dump ground and from Faculty of Agricultural Science farm land. The isolates were morphologically distinct on the basis of spore colour, aerial and substrate mycelium formation and production of diffusible pigment, Isolates were tested under a microscope by using  Gram staining technique they were all Gram negative, biochemical test was carried out the two isolate such as catalase test which was catalase positive and there was production of burbles, citrate test it was citrate positive and there was a change of colour to green blue and oxidase test  it was oxidase negative. One of the two isolates were observed to have significant antibiotic producing potential, and the antibacterial activity of the produced antibiotics (Red pigment extracted from cultural supernatants of the isolates grown on Pepton glycerol agar) was assessed using the well-in-agar diffusion method and streaking agar method. The results indicated that the extracted pigment had varying antibacterial activity at different concentration level of 500mg/ml, 250mg/ml 125mg/ml and 62.5mg/ml against 4 strains of the test organism (Staphylococcus aureus) titled S1 to S4, and the extracted pigment was more effective at concentration 500mg/ml against the antibiotic resistant Staphylococcus aureus. The results indicate that the soil of this region is source of prodigiocin having antibacterial activity and thus enable the use of micro-organisms as biological control agents.

 TABLE OF CONTENT

TITTLE PAGE

DECLERATION..............................................................................i

CERTIFICATION............................................................................ii

DEDICATION.................................................................................iii

ACKNOWLEDGEMENT................................................................iv

ABSTRACT......................................................................................v

TABLE OF CONTENT ....................................................................vi

LIST OF TABLES ............................................................................vii

 

CHAPTER ONE

Introduction…………………………………………………………

Justification…………………………………………………………

Aim…………………………………………………………………..

Objectives…………………………………………………………..

 

CHAPTER TWO

Literature review………………………………………………….

Antibiotics………………………………………………………..

Antibiotics resistances to staphylococcus aureus………………..

Antibiotics production from microorganism…………………….

Historical background…………………………………………….

Why an effort for antibiotics search……………………………….

Concrete objectives of antibiotics research………………………

The Mechanism of action of antibiotics…………………………..

Functions of soil microorganisms…………………………………

Some antibiotic producing microorganisms………………………

Serratia marcesciens……………………………………………..

Prodigiosin……………………………………………………….

 

CHAPTER THREE

Materials and methods…………………………………………….

Material list……………………………………………………….

Sterilisation of glass wears ………………………………………..

Antibiotic sensitivity assay ………………………………………

Soil sample collection ……………………………………………

Drying of soil sample…………………………………………….

Preparation of isolation media …………………………………..

Plating ……………………………………………………………

Identification of growth ………………………………………….

Morphological identification ……………………………………

Physiological identification ……………………………………..

Gram staining …………………………………………………..

Motility test ……………………………………………………

Biochemical identification ……………………………………

Oxidase test …………………………………………………...

Catalase test …………………………………………………

Citrate utilization test ………………………………………..

Media preparation ………………………………………….

Inoculation of medium ……………………………………..

Invitro prodigiosin production ……………………………..

Harvesting of pigment and evaporation to dryness…………..

Determination of minimum inhibitory………………………..

concentration (MIC) of extracted pigment

Antimicrobial activity of extracted red pigment………………

on clinical isolate

Statistical analysis …………………………………………….

 

CHAPTER FOUR

Results …………………………………………………………..

Staphylococcus aureus antibiotic resistance pattern ……………

Physical characteristics of soil samples collected ………………

Morphological characteristics ………………………………….

Biochemical characterisation of RM1 isolate …………………..

Minimum inhibitory concentration of the red…………………..

pigment produced by serratia marcescences

Antimicrobial Assay …………………………………………..

 

CHAPTER FIVE

5.0     Discussion and conclusion................................................................

5.1     Discussion..........................................................................................

5.2     Conclusion..........................................................................................

REFERENCE................................................................................................

APPENDIX 1.................................................................................................xi

APPENDIX 2..................................................................................................xvi

APPENDIX 3..................................................................................................xviii

APPENDIX 4...................................................................................................xxi

STATISTICAL ANALYSIS

Data Analysis....................................................................................................xiii

Introduction ......................................................................................................xiii

Analysis............................................................................................................xiii

Analysis of Variance (ANOVA).....................................................................xiii

To test the effect of extract on S.aureus .............xiii

Descriptive Statistics.......................................................................................xiv

 

GRAPHICAL REPRESENTATION

LIST OF Tables

Antibiotic sensitivity and resistance pattern…………………………….

of four strains of S.aureus against Oxacilin

Characterization of soil samples collect …………………………………

Cultural characteristics of soil isolates ……………………………………

Biochemical characterization of RMN1…………………………………..

Minimum inhibitory concentration (MIC)………………………………..

of Red pigment produced by S.marsceinces  

Antimicrobial activities of red pigment extracted ……………………….

Appendix

Analysis of variance………………………………………………….

Descriptive statistics …………………………………………

Summary ………………………………………………………

  

Appendix

Pictorial Presentations of Plates and Microscopy

Pictorial Presentations of Plates

 

 

 

 

 

 

 

 

 

 

 

 


ISOLATION OF ANTIBIOTIC PRODUCING STREPTOMYCES FROM THE SOIL AS SOURCE ANTIBIOTICS ACTIVE AGAINST ANTIBIOTICRESISTANT STAPHYLOCOCCUS AUREUS
For more Info, call us on
+234 8130 686 500
or
+234 8093 423 853

Share This
  • Type: Project
  • Department: Microbiology
  • Project ID: MCB0189
  • Access Fee: ₦5,000 ($14)
  • Pages: 60 Pages
  • Format: Microsoft Word
  • Views: 1K
Payment Instruction
Bank payment for Nigerians, Make a payment of ₦ 5,000 to

Bank GTBANK
gtbank
Account Name Obiaks Business Venture
Account Number 0211074565

Bitcoin: Make a payment of 0.0005 to

Bitcoin(Btc)

btc wallet
Copy to clipboard Copy text

500
Leave a comment...

    Details

    Type Project
    Department Microbiology
    Project ID MCB0189
    Fee ₦5,000 ($14)
    No of Pages 60 Pages
    Format Microsoft Word

    Related Works

    ABSTRACT This research work involve the isolation of two isolates titled RMN1 and RMN2, belonging to the Genus Serratia from two soil samples collected from the following two strategic locations in university of Abuja, permanent site: Termite hill besides Girls hostel dump ground and from Faculty of Agricultural Science farm land. The isolates... Continue Reading
      ABSTRACT This research work involve the isolation of two isolates titled RMN1 and RMN2, belonging to the Genus Serratia  from two soil samples collected from the following two strategic locations in university of Abuja, permanent site: Termite hill besides Girls hostel dump ground and from Faculty of Agricultural Science farm land. The... Continue Reading
    ABSTRACT Isolation and characterisatics of an antibiotics producing bacterium collected from the soil near the arbatior in artesan market Enugu was achieved by characterizing a collection of 74 soil bacteria, including eubacteria and actinomycetes, according to their ability to produce antibacterial antibiotics and their growth responses to 43... Continue Reading
    ABSTRACT Isolation and characterisatics of an antibiotics producing bacterium collected from the soil near the arbatior in artesan market Enugu was achieved by characterizing a collection of 74 soil bacteria, including eubacteria and actinomycetes, according to their ability to produce antibacterial antibiotics and their growth responses to 43... Continue Reading
    ABSTRACT        This work was done to determine the antibiotic susceptibility of Staphylococcus aureus among cattle in Owerri, Imo State. A total of twenty four (24) samples were collected which were cultured on appropriate bacteriological media. The Staphylococcus aureus isolates were identified by standard biochemical tests. Antibiotic... Continue Reading
    ABSTRACT This work was done to determine the antibiotic susceptibility of Staphylococcus aureus among cattle in Owerri, Imo State. A total of twenty four (24) samples were collected which were cultured on appropriate bacteriological media. The Staphylococcus aureus isolates were identified by standard biochemical tests. Antibiotic susceptibility... Continue Reading
    ABSTRACT Back ground: Staphylococcus aureus is a gram positive bacteria that exists on the skin as nonnal flora. It is classified as Methicillin-resistant Staphylococcus aureus (MRSA) and Methicillin-sensitive Staphylococcus aureus (MSSA), where MRSA is resistant to beta-lactam antibiotics. MRSA has become a predominant pathogen in the health care... Continue Reading
    ABSTRACT A total of 100 subjects were screened for  S. aureus strains, thirty (30) S. aureus isolates were obatained. The carriage of β-lactamase among the 30 isolates was determined by using starch iodine agar plate method. It was found that 19(63.3%) of the S. aureus strains were β-lactamase producing. Plasmid curing of the β-lactamase... Continue Reading
    ABSTRACT A total of 100 subjects were screened for  S. aureus strains, thirty (30) S. aureus isolates were obatained. The carriage of β-lactamase among the 30 isolates was determined by using starch iodine agar plate method. It was found that 19(63.3%) of the S. aureus strains were β-lactamase producing. Plasmid curing of the β-lactamase... Continue Reading
    Abstract The study was carried out with aim to isolate  Staphylococcus aureus  from cow meat and determine the antibiogram pattern of  S. aureus . Three samples of cow meat from three different locations (Gwagwalada market, Abattoir market and  Kasuan Dare ) in Gwagwalada were collected to isolate  S. aureus  from the samples. The organisms... Continue Reading
    Call Us
    whatsappWhatsApp Us